Anticorps Polyclonal de lapin anti-PTEN

• Phare
• Validé par KD/KO

PTEN Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

152

N° de cat : 22034-1-AP

Synonymes

10q23del, Bannayan Zonana syndrome, BC005821, BZS, ITGA 2, MGC11227, MHAM, MMAC1, Multiple hamartoma (Cowden syndrome), phosphatase and tensin homolog, PTEN, PTEN1, TEP1

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Galerie de données de validation

• 

  WB analysis using 22034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22034-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 22034-1-PBS

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 22034-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22034-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22034-1-AP (PTEN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of DU 145 using 22034-1-AP

  IP result of anti-PTEN (IP:22034-1-AP, 4ug; Detection:22034-1-AP 1:500) with DU 145 cells lysate 1800 ug.

• 

  IHC staining of human breast cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 22034-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22034-1-AP (PTEN Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HeLa using 22034-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PTEN (22034-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules C6, cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules LNCaP, cellules MCF-7, cellules NIH/3T3, tissu de thymus de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules DU 145,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de souris, tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 17 publications below
• WB: See 138 publications below
• IHC: See 29 publications below
• IF: See 14 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below

Informations sur le produit

22034-1-AP cible PTEN dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTEN Protéine recombinante Ag17274
• Nom complet: phosphatase and tensin homolog
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 55 kDa, 68 kDa
• Numéro d’acquisition GenBank: BC005821
• Symbole du gène: PTEN
• Identification du gène (NCBI): 5728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phosphatase and tensin homolog (PTEN) is an enzyme key in cell cycle regulation and in tumor suppression.

What is the molecular weight of PTEN?

PTEN is composed of 403 amino acid residues with an N-terminal domain with phosphatase activity and a 

C-terminal C2 domain that binds phospholipid membranes (PMID:14749127). This antibody can recognize 

two isoforms with MWs of 55kDa and 68 kDa.

What is the function of PTEN?

Widely expressed throughout the body, PTEN is found in the cytoplasm and inhibits cell cycle progression 

through phosphatase activity. It regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate (PIP3) 

by catalyzing the dephosphorylation of the inositol ring. This function counteracts the growth-stimulating 

activity of the PI3K/AKT signaling pathway and thereby suppresses proliferation. Therefore, in homeostasis, 

PTEN promotes chromosome stability and DNA repair (PMID: 24387334).Expression and function can be 

regulated by posttranslational modifications, including acetylation for decreased catalytic activity and 

ubiquitination for protein degradation, nuclear localization, and inhibition of phosphatase activity 

(PMID: 24656806).

What is the role of PTEN in cancer?

PTEN was first identified as a tumor suppressor when mutations or deletions were identified by two separate 

groups in a number of different cancer types including gliomas, breast, prostate, and kidney cancer 

(PMID: 9090379; PMID: 9072974). Non-inherited, or somatic, mutations in PTEN that lead to a loss of function 

in the protein have been shown to drive malignancy in tumors. PTEN hamartoma tumor syndrome (PHTS) is a 

group of inherited disorders that occur as a result of autosomal dominant mutations in the PTEN gene. These 

disorders include Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and Proteus syndrome. Because of 

the constitutive expression of PTEN, the symptoms are varied but often include neurodevelopmental issues and 

an increased risk of developing tumors, both benign and malignant (PMID: 26564076).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Myo9b mutations are associated with altered dendritic cell functions and increased susceptibility to autoimmune diabetes onset; Authors - Jing Zhang
human	WB	Biomater Res; High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration; Authors - Di Sun
human,mouse	IHC,WB	Drug Des Devel Ther; Network Pharmacology and Experimental Validation to Explore That Celastrol Targeting PTEN is the Potential Mechanism of Tripterygium wilfordii (Lév.) Hutch Against IgA Nephropathy; Authors - Juanyong Zhao
mouse	WB	J Pineal Res; Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload; Authors - Xinghua Qin
mouse	WB	Theranostics; Histone demethylase JMJD3 protects against renal fibrosis by suppressing TGFβ and Notch signaling and preserving PTEN expression.; Authors - Chao Yu
human	WB,IHC,IF	Cell Prolif; YTHDC1 positively regulates PTEN expression and plays a critical role in cisplatin resistance of bladder cancer; Authors - Yinjie Su

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