Anticorps Recombinant de lapin anti-PTEN

PTEN Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240134F7

N° de cat : 80718-7-PBS

Synonymes

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

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Galerie de données de validation

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  IF Staining of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTEN antibody (80718-7-RR, Clone: 240134F7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PTEN Recombinant antibody (80718-7-RR, Clone:240134F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80718-7-PBS

  Biolayer interferometry (BLl) kinetic assays of 80718-7-RR against Human PTEN were performed. The affinity constant is below 1 pM.

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Informations sur le produit

80718-7-PBS cible PTEN dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PTEN Protéine recombinante Ag17274
• Nom complet: phosphatase and tensin homolog
• Masse moléculaire calculée: 47 kDa
• Numéro d’acquisition GenBank: BC005821
• Symbole du gène: PTEN
• Identification du gène (NCBI): 5728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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