Anticorps Recombinant de lapin anti-PTGES2

PTGES2 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242930B4

N° de cat : 85419-4-PBS

Synonymes

242930B4, C9orf15, EC:5.3.99.3, GBF1, Membrane-associated prostaglandin E synthase-2

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Galerie de données de validation

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  WB analysis using 85419-4-RR (same clone as 85419-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

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  WB analysis using 85419-4-RR (same clone as 85419-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85419-4-RR (same clone as 85419-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85419-4-RR (same clone as 85419-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85419-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85419-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85419-4-RR against Human PTGES2 were performed. The affinity constant is 0.973 nM.

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Informations sur le produit

85419-4-PBS cible PTGES2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PTGES2 Protéine recombinante Ag1329
• Nom complet: prostaglandin E synthase 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC011613
• Symbole du gène: PTGES2
• Identification du gène (NCBI): 80142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).

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