Anticorps Polyclonal de lapin anti-PTGES3

PTGES3 Polyclonal Antibody for FC, IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15216-1-AP

Synonymes

cPGES, Hsp90 co chaperone, P23, Prostaglandin E synthase 3, PTGES3, TEBP, Telomerase binding protein p23

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Galerie de données de validation

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  WB analysis of rat brain using 15216-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15216-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15216-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 15216-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15216-1-AP (PTGES3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15216-1-AP

  IP Result of anti-PTGES3 (IP:15216-1-AP, 3ug; Detection:15216-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IF Staining of A549 using 15216-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 15216-1-AP (PTGES3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15216-1-AP

  Immunofluorescent analysis of HeLa cells using 15216-1-AP (PTGES3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 15216-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PTGES3 antibody (15216-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IF: cellules A549, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

15216-1-AP cible PTGES3 dans les applications de WB, IP, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTGES3 Protéine recombinante Ag7384
• Nom complet: prostaglandin E synthase 3 (cytosolic)
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 21-23 kDa
• Numéro d’acquisition GenBank: BC003005
• Symbole du gène: PTGES3
• Identification du gène (NCBI): 10728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).

Publications

Species	Application	Title
mouse	WB	Cell Death Differ; Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis.; Authors - Yujia Fan
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
mouse	WB	Heliyon; The inhibitory effects of Orengedokuto on inducible PGE2 production in BV-2 microglial cells.; Authors - Yoshika Iwata
human	WB	Sci Adv; PES1 is a critical component of telomerase assembly and regulates cellular senescence.; Authors - Long Cheng