Anticorps Monoclonal anti-PTGES3

PTGES3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

3C11D11

N° de cat : 67736-1-Ig

Synonymes

cPGES, Hsp90 co chaperone, P23, Prostaglandin E synthase 3, PTGES3, TEBP, Telomerase binding protein p23

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Galerie de données de validation

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  WB analysis using 67736-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67736-1-Ig (PTGES3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67736-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67736-1-Ig (PTGES3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67736-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67736-1-Ig (PTGES3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67736-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES3 antibody (67736-1-Ig, Clone: 3C11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67736-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES3 antibody (67736-1-Ig, Clone: 3C11D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules NIH/3T3, cellules PC-3, tissu cérébral de porc
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

67736-1-Ig cible PTGES3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTGES3 Protéine recombinante Ag7870
• Nom complet: prostaglandin E synthase 3 (cytosolic)
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003005
• Symbole du gène: PTGES3
• Identification du gène (NCBI): 10728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; High expression of PTGES3 is an independent predictive poor prognostic biomarker and correlates with immune infiltrates in lung adenocarcinoma.; Authors - Pengxiang Gao
rat	IF	Curr Pharm Des; Integrated Metabolomics and Proteomics to Decipher Simiao Pill Improving Lipid Homeostasis through PTGES3-mediated Arachidonic Acid Metabolism in AIA Model; Authors - Ying Cai
human	WB	Cell Death Discov; METTL9-SLC7A11 axis promotes hepatocellular carcinoma progression through ferroptosis inhibition; Authors - Fangfang Bi
human	WB	J Transl Med; The integration of multidisciplinary approaches revealed PTGES3 as a novel drug target for breast cancer treatment; Authors - Qinan Yin
human	WB,IHC	Reprod Sci; Progressively Diminished Prostaglandin E2 Signaling in Concordance with Increasing Fibrosis in Ectopic Endometrium; Authors - Yunhua Yi
human	IHC	Biol Direct; PTGES3 proteolysis using the liposomal peptide-PROTAC approach; Authors - Shiwei Liu

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