Anticorps Polyclonal de lapin anti-PTGR1

PTGR1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13374-1-AP

Synonymes

LTB4DH, PGR1, PRG 1, prostaglandin reductase 1, PTGR1, ZADH3

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Galerie de données de validation

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  WB analysis using 13374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13374-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 13374-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13374-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13374-1-AP

  IP result of anti-PTGR1 (IP:13374-1-AP, 4ug; Detection:13374-1-AP 1:500) with HepG2 cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu de côlon humain, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

13374-1-AP cible PTGR1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTGR1 Protéine recombinante Ag4196
• Nom complet: prostaglandin reductase 1
• Masse moléculaire calculée: 329 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC035228
• Symbole du gène: PTGR1
• Identification du gène (NCBI): 22949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTGR1(Prostaglandin reductase 1) is also named as LTB4DH(NADP-dependent leukotriene B4 12-hydroxydehydrogenase) and belongs to the NADP-dependent oxidoreductase L4BD family. It catalyses the conversion of leukotriene B4 into biologically less reactive 12-oxo-leukotriene B4, a key step for its inactivation and it is a key oxidation step in the process of metabolic inactivation of leukotriene B(4) in various non-leukocyte tissues(PMID:8576264).

Publications

Species	Application	Title
human	WB	J Biol Chem; Modulation of Nitro-Fatty Acid Signaling: Prostaglandin Reductase-1 is a Nitroalkene Reductase.; Authors - Vitturi Dario A DA
human	WB	Mol Cancer Ther; Conditional Dependency of LP-184 on Prostaglandin Reductase 1 is Synthetic Lethal in Pancreatic Cancers with DNA Damage Repair Deficiencies; Authors - Diana Restifo