Anticorps Polyclonal de lapin anti-COX-1/Cyclooxygenase-1

• Phare
• Validé par KD/KO

COX-1/Cyclooxygenase-1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(32)

Avis (1)

N° de cat : 13393-1-AP

Synonymes

PGH1, PTGS1, COX1, Cyclooxygenase-1, EC:1.14.99.1

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Galerie de données de validation

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  WB analysis of A431 using 13393-1-AP

  WB result of COX-1/Cyclooxygenase-1 antibody (13393-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX-1/Cyclooxygenase-1 transfected A431 cells.

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  WB analysis using 13393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 13393-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13393-1-AP

  IP result of anti-COX-1/Cyclooxygenase-1 (IP:13393-1-AP, 4ug; Detection:13393-1-AP 1:500) with HeLa cells lysate 1280 ug.

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  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 13393-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13393-1-AP (COX-1/Cyclooxygenase-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules C2C12, cellules HeLa, cellules Neuro-2a, cellules THP-1
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 31 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13393-1-AP cible COX-1/Cyclooxygenase-1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX-1/Cyclooxygenase-1 Protéine recombinante Ag4055
• Nom complet: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• Masse moléculaire calculée: 599 aa, 69 kDa
• Poids moléculaire observé: 60-72 kDa
• Numéro d’acquisition GenBank: BC029840
• Symbole du gène: PTGS1
• Identification du gène (NCBI): 5742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

Publications

Species	Application	Title
rat	WB	Acta Pharm Sin B; Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.; Authors - Dominik Bakalarz
	WB	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
mouse	WB	Theranostics; Nanozyme-mediated catalytic nanotherapy for inflammatory bowel disease.; Authors - Jiulong Zhao
rat	WB	Elife; Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control; Authors - Meiling Wu
human	WB	Free Radic Biol Med; PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis; Authors - Qiong Sun
mouse	WB	Ecotoxicol Environ Saf; Exposure to nicotine regulates prostaglandin E2 secretion and autophagy of granulosa cells to retard follicular maturation in mammals; Authors - Xiaofeng Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Thomas (Verified Customer) (09-18-2020)

HeLa cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 488 Alexa Fluor secondary antibody (1:250 - green) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HeLa

COX-1/Cyclooxygenase-1 Antibody Immunofluorescence validation (1:200 dilution) in HeLa (Cat no:13393-1-AP)

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