• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-COX-1/Cyclooxygenase-1

COX-1/Cyclooxygenase-1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 13393-1-AP

Synonymes

COX1, COX-1, COX-1/Cyclooxygenase-1, COX3, Cyclooxygenase 1, PCOX1, PGG/HS, PGH synthase 1, PGHS 1, PGHS1, PHS 1, PHS1, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, PTGHS, PTGS1



Applications testées

Résultats positifs en WBcellules HeLa, cellule C2C12, cellules Neuro-2a
Résultats positifs en IPcellules HeLa,
Résultats positifs en IHCtissu d'estomac humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

13393-1-AP cible COX-1/Cyclooxygenase-1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Chèvre, Humain, porc, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène COX-1/Cyclooxygenase-1 Protéine recombinante Ag4055
Nom complet prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Masse moléculaire calculée 599 aa, 69 kDa
Poids moléculaire observé 60-72 kDa
Numéro d’acquisition GenBankBC029840
Symbole du gène PTGS1
Identification du gène (NCBI) 5742
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

Protocole

Product Specific Protocols
WB protocol for COX-1/Cyclooxygenase-1 antibody 13393-1-APDownload protocol
IHC protocol for COX-1/Cyclooxygenase-1 antibody 13393-1-APDownload protocol
IP protocol for COX-1/Cyclooxygenase-1 antibody 13393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Acta Pharm Sin B

Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.

Authors - Dominik Bakalarz
WB

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
mouseWB

Theranostics

Nanozyme-mediated catalytic nanotherapy for inflammatory bowel disease.

Authors - Jiulong Zhao
ratWB

Elife

Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control

Authors - Meiling Wu
humanWB

Free Radic Biol Med

PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis

Authors - Qiong Sun
humanWB

Acta Biomater

Localized delivery of miRNAs targets cyclooxygenases and reduces flexor tendon adhesions.

Authors - You Lang Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Thomas (Verified Customer) (09-18-2020)

HeLa cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 488 Alexa Fluor secondary antibody (1:250 - green) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: HeLa
COX-1/Cyclooxygenase-1 Antibody Immunofluorescence validation (1:200 dilution) in HeLa (Cat no:13393-1-AP)