Anticorps Monoclonal anti-PYK2

PYK2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A10G5

N° de cat : 67141-1-PBS

Synonymes

PTK2B, 2A10G5, CADTK, CAK beta, CAKB

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Galerie de données de validation

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  WB analysis using 67141-1-Ig (same clone as 67141-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PYK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PYK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67141-1-Ig (PYK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67141-1-Ig (PYK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67141-1-PBS cible PYK2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PYK2 Protéine recombinante Ag11487
• Nom complet: PTK2B protein tyrosine kinase 2 beta
• Masse moléculaire calculée: 1009 aa, 116 kDa
• Poids moléculaire observé: 112-115 kDa
• Numéro d’acquisition GenBank: BC042599
• Symbole du gène: PTK2B
• Identification du gène (NCBI): 2185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.

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