Anticorps Polyclonal de lapin anti-PTK7/CCK4

• Phare
• Validé par KD/KO

PTK7/CCK4 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 17799-1-AP

Synonymes

PTK7, Protein tyrosine kinase 7, Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, CCK-4

---

Galerie de données de validation

• 

  WB analysis using 17799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17799-1-AP (PTK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PaTu 8988s using 17799-1-AP

  WB result of PTK7 antibody (17799-1-AP; 1:2000; room temperature for 1.5 hours) with wild-type and PTK7 knockout PaTu 8988s cells.

• 

  WB analysis of Jurkat using 17799-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17799-1-AP (PTK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17799-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17799-1-AP (PTK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 17799-1-AP

  IP result of anti-PTK7 (IP:17799-1-AP, 4ug; Detection:17799-1-AP 1:300) with Jurkat cells lysate 3400ug.

• 

  IHC staining of human colon cancer using 17799-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17799-1-AP (PTK7 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17799-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17799-1-AP (PTK7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17799-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17799-1-AP (PTK7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules PC-12, cellules A431, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

17799-1-AP cible PTK7/CCK4 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTK7/CCK4 Protéine recombinante Ag12004
• Nom complet: PTK7 protein tyrosine kinase 7
• Masse moléculaire calculée: 1070 aa, 118 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC071557
• Symbole du gène: PTK7
• Identification du gène (NCBI): 5754
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is an atypical receptor tyrosine kinase that consists of an extracellular domain with seven immunoglobulin-like domains, a transmembrane domain, and a catalytically defective tyrosine kinase domain (PMID: 29867084). It is implicated in planar cell polarity and in the Wnt canonical and non-canonical pathways (PMID: 24618420). Overexpression of PTK7 has been reported in a variety of tumors, such as cervical cancer, colon cancer, lung adenocarcinoma, acute myelogenous leukemia, and gastric cancer (PMID: 30944666).

Publications

Species	Application	Title
Human	WB,IP,IHC,IF	Cell Death Dis; Periostin secreted by cancer-associated fibroblasts promotes cancer stemness in head and neck cancer by activating protein tyrosine kinase 7.; Authors - Binbin Yu
human	IHC	Mod Pathol; USCAP 2018 Abstracts: Techniques (2229-2304).; Authors - W. D. Mojica
mouse	WB	Life Sci; Identification of metastasis-related genes by genomic and transcriptomic studies in murine melanoma.; Authors - Onat Kadioglu
human	WB	Med Sci Monit; Bioinformatics Analysis Identifies Protein Tyrosine Kinase 7 (PTK7) as a Potential Prognostic and Therapeutic Biomarker in Stages I to IV Hepatocellular Carcinoma.; Authors - Ren-Chao Zou
human	IHC	Clin Exp Dent Res; High expression of protein tyrosine kinase 7 in oral squamous cell carcinoma: Clinicopathological correlation and prognosis relevance.; Authors - Yujiro Kimura
mouse	IF	Cell Rep; Phosphatidylinositol transfer protein/planar cell polarity axis regulates neocortical morphogenesis by supporting interkinetic nuclear migration.; Authors - Zhigang Xie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Gabriel (Verified Customer) (01-15-2019)

• Applications: Immunoprecipitation,
• Cell Tissue Type: MCF10A

PTK7/CCK4 Antibody Immunoprecipitation, validation ( dilution) in MCF10A (Cat no:17799-1-AP)

{{ptg:RelatedPrimaryAntibodies}}