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Anticorps Recombinant de lapin anti-PTPN1/PTP1B

PTPN1/PTP1B Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250215G6

N° de cat : 85905-4-PBS

Synonymes

PTP1B, PTPN1, EC:3.1.3.48, Protein-tyrosine phosphatase 1B, PTP 1B

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using PTPN1/PTP1B Recombinant monoclonal antibody (85905-4-RR)

    WB analysis using 85905-4-RR (same clone as 85905-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85905-4-RR (PTPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85905-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTPN1/PTP1B Recombinant monoclonal antibody (85905-4-RR)

    IF Staining of HeLa using 85905-4-RR (same clone as 85905-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPN1 antibody (85905-4-RR, Clone: 250215G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85905-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85905-4-RR

    Affinity and Kinetic Characterization of 85905-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85905-4-RR against Human PTPN1/PTP1B were performed. The affinity constant is below 1 pM.

Informations sur le produit

85905-4-PBS cible PTPN1/PTP1B dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène PTPN1/PTP1B Protéine recombinante Ag1878
Nom complet protein tyrosine phosphatase, non-receptor type 1
Masse moléculaire calculée 435 aa, 50 kDa
Poids moléculaire observé50 kDa
Numéro d’acquisition GenBankBC015660
Symbole du gène PTP1B
Identification du gène (NCBI) 5770
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

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