Anticorps Recombinant de lapin anti-PTPN1/PTP1B

PTPN1/PTP1B Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250215G6

N° de cat : 85905-4-RR

Synonymes

PTP1B, PTPN1, EC:3.1.3.48, Protein-tyrosine phosphatase 1B, PTP 1B

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Galerie de données de validation

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  WB analysis of MCF-7 using 85905-4-RR

  WB result of PTPN1/PTP1B antibody (85905-4-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN1/PTP1B transfected MCF-7 cells.

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  WB analysis using 85905-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85905-4-RR (PTPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85905-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPN1 antibody (85905-4-RR, Clone: 250215G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85905-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85905-4-RR against Human PTPN1/PTP1B were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules Jurkat, cellules THP-1
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85905-4-RR cible PTPN1/PTP1B dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PTPN1/PTP1B Protéine recombinante Ag1878
• Nom complet: protein tyrosine phosphatase, non-receptor type 1
• Masse moléculaire calculée: 435 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC015660
• Symbole du gène: PTP1B
• Identification du gène (NCBI): 5770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

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