Anticorps Polyclonal de lapin anti-SHP2

SHP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24570-1-PBS

Synonymes

PTPN11, NS1, NS 1, EC:3.1.3.48, CFC

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 24570-1-AP (same clone as 24570-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24570-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 24570-1-AP (same clone as 24570-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24570-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 24570-1-AP (same clone as 24570-1-PBS)

  WB result of PTPN11 antibody (24570-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN11 transfected HeLa cells. This data was developed using the same antibody clone with 24570-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 24570-1-AP (same clone as 24570-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 24570-1-AP (same clone as 24570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 24570-1-AP (same clone as 24570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 24570-1-AP (same clone as 24570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24570-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 24570-1-AP (same clone as 24570-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24570-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 24570-1-AP (same clone as 24570-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11/SHP2 antibody (24570-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 24570-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

24570-1-PBS cible SHP2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHP2 Protéine recombinante Ag21472
• Nom complet: protein tyrosine phosphatase, non-receptor type 11
• Masse moléculaire calculée: 597 aa, 68 kDa
• Poids moléculaire observé: 68 kda
• Numéro d’acquisition GenBank: BC008692
• Symbole du gène: PTPN11
• Identification du gène (NCBI): 5781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}