Anticorps Recombinant de lapin anti-SHP2

• Phare
• Validé par KD/KO

SHP2 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5L23

N° de cat : 82503-1-PBS

Synonymes

PTPN11/SHP2, PTPN11, NS 1, EC:3.1.3.48, CFC

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Galerie de données de validation

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  WB analysis using 82503-1-RR (same clone as 82503-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82503-1-RR (PTPN11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82503-1-RR (same clone as 82503-1-PBS)

  WB result of SHP2 antibody (82503-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHP2 transfected HeLa cells. This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 82503-1-RR (same clone as 82503-1-PBS)

  IP result of anti-PTPN11/SHP2 (IP:82503-1-RR, 4ug; Detection:82503-1-RR 1:8000) with HEK-293 cells lysate 1280 ug. This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 82503-1-RR (same clone as 82503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 82503-1-RR (same clone as 82503-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82503-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82503-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82503-1-RR against Human PTPN11/SHP2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82503-1-PBS cible SHP2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SHP2 Protéine recombinante Ag13660
• Nom complet: protein tyrosine phosphatase, non-receptor type 11
• Masse moléculaire calculée: 597 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC008692
• Symbole du gène: PTPN11
• Identification du gène (NCBI): 5781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTPN11(protein tyrosine phosphatase, non-receptor type 11), also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, and MGC14433, belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML), and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

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