Anticorps Polyclonal de lapin anti-PTPN12

PTPN12 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31215-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 31215-1-AP (same clone as 31215-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31215-1-AP (PTPN12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31215-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 31215-1-AP (same clone as 31215-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31215-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 31215-1-AP (same clone as 31215-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31215-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31215-1-PBS cible PTPN12 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPN12 Protéine recombinante Ag35382
• Nom complet: protein tyrosine phosphatase, non-receptor type 12
• Masse moléculaire calculée: 88kDa
• Poids moléculaire observé: 110-125 kDa
• Numéro d’acquisition GenBank: NM_002835.3
• Symbole du gène: PTPN12
• Identification du gène (NCBI): 5782
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.

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