Anticorps Polyclonal de lapin anti-PTPN14

• Phare
• Validé par KD/KO

PTPN14 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 18053-1-AP

Synonymes

PEZ, PTP36, PTPN14

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Galerie de données de validation

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  WB analysis using 18053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18053-1-AP

  WB result of PTPN14 antibody (18053-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN14 transfected HeLa cells.

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  WB analysis of HeLa using 18053-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18053-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 18053-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562
• Résultats positifs en IHC: tissu placentaire humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

18053-1-AP cible PTPN14 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPN14 Protéine recombinante Ag12571
• Nom complet: protein tyrosine phosphatase, non-receptor type 14
• Masse moléculaire calculée: 1187 aa, 135 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC101754
• Symbole du gène: PTPN14
• Identification du gène (NCBI): 5784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

Publications

Species	Application	Title
canine	WB	Biologicals; CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells; Authors - Zhenbin Liu