Anticorps Monoclonal anti-PTPN14

PTPN14 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B8C8

N° de cat : 67744-1-PBS

Synonymes

1B8C8, EC:3.1.3.48, PEZ, Protein-tyrosine phosphatase pez, PTP 36

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Galerie de données de validation

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  WB analysis using 67744-1-Ig (same clone as 67744-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67744-1-Ig (PTPN14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67744-1-Ig (same clone as 67744-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTPN14 antibody (67744-1-Ig, Clone: 1B8C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67744-1-PBS cible PTPN14 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTPN14 Protéine recombinante Ag12679
• Nom complet: protein tyrosine phosphatase, non-receptor type 14
• Masse moléculaire calculée: 1187 aa, 135 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC101754
• Symbole du gène: PTPN14
• Identification du gène (NCBI): 5784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

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