Anticorps Recombinant de lapin anti-PTPN18

PTPN18 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230154G8

N° de cat : 83024-1-PBS

Synonymes

Tyrosine-protein phosphatase non-receptor type 18, PTP HSCF, EC:3.1.3.48, Brain-derived phosphatase, BDP1

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Galerie de données de validation

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  WB analysis using 83024-1-RR (same clone as 83024-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 83024-1-RR (PTPN18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83024-1-PBS in a different storage buffer formulation.

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  WB analysis using 83024-1-RR (same clone as 83024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83024-1-RR (PTPN18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83024-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 83024-1-RR (same clone as 83024-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PTPN18 Recombinant antibody (83024-1-RR, Clone:230154G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83024-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83024-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83024-1-RR against Human PTPN18 were performed. The affinity constant is 7.48 nM.

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Informations sur le produit

83024-1-PBS cible PTPN18 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PTPN18 Protéine recombinante Ag34048
• Nom complet: protein tyrosine phosphatase, non-receptor type 18 (brain-derived)
• Masse moléculaire calculée: 460 aa, 50 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC052800
• Symbole du gène: PTPN18
• Identification du gène (NCBI): 26469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTPN18 (also called PTP-HSCF or BDP1), a member of the protein tyrosine phosphatase superfamily, has been reported to be a tumor suppressor in breast cancer by dephosphorylating HER2 and suppressing cell proliferation and invasion. In addition to the well-established role of PTPN18 in breast cancer, our previous study found that PTPN18 and CSK cooperate to inhibit the events triggered by SRC kinases. (PMID: 35982039)

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