Anticorps Polyclonal de lapin anti-PTPN2

• Phare
• Validé par KD/KO

PTPN2 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 11214-1-AP

Synonymes

PTPN2, PTPT, TC PTP, TCELLPTP, TCPTP

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Galerie de données de validation

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  WB analysis of mouse thymus using 11214-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11214-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11214-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11214-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11214-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11214-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 11214-1-AP

  IP Result of anti-PTPN2 (IP:11214-1-AP, 4ug; Detection:11214-1-AP 1:500) with HL-60 cells lysate 4000ug.

• 

  IHC staining of human colon cancer using 11214-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11214-1-AP (PTPN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11214-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 11214-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules HeLa, cellules HL-60, cellules NIH/3T3
• Résultats positifs en IP: cellules HL-60
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 6 publications below
• IHC: See 7 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

11214-1-AP cible PTPN2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPN2 Protéine recombinante Ag1709
• Nom complet: protein tyrosine phosphatase, non-receptor type 2
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC016727
• Symbole du gène: PTPN2
• Identification du gène (NCBI): 5771
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

Publications

Species	Application	Title
mouse	IHC	Oncogene; Overexpression of TC-PTP in murine epidermis attenuates skin tumor formation.; Authors - Mihwa Kim
human,mouse	WB,IHC	Int Immunopharmacol; Hypoxia disrupts the nasal epithelial barrier by inhibiting PTPN2 in chronic rhinosinusitis with nasal polyps; Authors - Meiping Zhang ; KD Validated
mouse	IHC	Int Immunopharmacol; Oral squamous cell carcinoma-derived EVs promote tumor progression by regulating inflammatory cytokines and the IL-17A-induced signaling pathway; Authors - Ruowei Li
human	WB	Cell Death Dis; Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation.; Authors - Liang Lu ; KD Validated
mouse	WB,IHC	Sci Rep; Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development.; Authors - Hyunseung Lee ; KO Validated
human	IF	Infect Immun; An Ehrlichia chaffeensis tandem repeat protein interacts with multiple host targets involved in cell signaling, transcriptional regulation, and vesicle trafficking.; Authors - Wakeel Abdul A