Anticorps Polyclonal de lapin anti-PTPN22

PTPN22 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11783-1-PBS

Synonymes

EC:3.1.3.48, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, LyP, LYP1, LYP2

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Galerie de données de validation

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  WB analysis using 11783-1-AP (same clone as 11783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11783-1-AP (same clone as 11783-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

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  WB analysis using 11783-1-AP (same clone as 11783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 11783-1-AP (same clone as 11783-1-PBS)

  IP result of anti-PTPN22 (IP:11783-1-AP, 4ug; Detection:11783-1-AP 1:300) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11783-1-AP (same clone as 11783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11783-1-AP (PTPN22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11783-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11783-1-PBS cible PTPN22 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPN22 Protéine recombinante Ag2397
• Nom complet: protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
• Masse moléculaire calculée: 82 kDa, 92 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC017785
• Symbole du gène: PTPN22
• Identification du gène (NCBI): 26191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.

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