Anticorps Polyclonal de lapin anti-PTPN7

PTPN7 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15286-1-AP

Synonymes

BPTP 4, HEPTP, LC PTP, LPTP, PTPN7, PTPNI

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Galerie de données de validation

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  WB analysis using 15286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15286-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15286-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15286-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules K-562
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

15286-1-AP cible PTPN7 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPN7 Protéine recombinante Ag7453
• Nom complet: protein tyrosine phosphatase, non-receptor type 7
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC001746
• Symbole du gène: PTPN7
• Identification du gène (NCBI): 5778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPN7, also known as haematopoietic PTP (HePTP), belongs to the R7 family of protein tyrosine phosphatases (PTPs) which all contain a conserved 16 amino acid sequence at their N-termini (PMID: 9857190). PTPN7, via negative regulation of MAPK signalling, plays a key role in T-cell and B-cell development and is the only known PTP whose expression in T-lymphocytes is induced by IL-2 (PMID: 15980098). PTPN7 is a 38 kDa non-receptor PTP expressed predominately in leukocytes, spleen and thymus (PMID: 1530918, 24265715, 19636879).

Publications

Species	Application	Title
pig	WB	Front Cell Dev Biol; piR-121380 Is Involved in Cryo-Capacitation and Regulates Post-Thawed Boar Sperm Quality Through Phosphorylation of ERK2 via Targeting PTPN7.; Authors - Yihan Wang
human	IHC	Front Genet; Comprehensive analysis of PTPN gene family revealing PTPN7 as a novel biomarker for immuno-hot tumors in breast cancer; Authors - Fengxu Wang