Anticorps Polyclonal de lapin anti-PTPN7

PTPN7 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 15286-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15286-1-AP (same clone as 15286-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 15286-1-AP (same clone as 15286-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 15286-1-AP (same clone as 15286-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 15286-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15286-1-PBS cible PTPN7 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPN7 Protéine recombinante Ag7453
• Nom complet: protein tyrosine phosphatase, non-receptor type 7
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC001746
• Symbole du gène: PTPN7
• Identification du gène (NCBI): 5778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTPN7, also known as haematopoietic PTP (HePTP), belongs to the R7 family of protein tyrosine phosphatases (PTPs) which all contain a conserved 16 amino acid sequence at their N-termini (PMID: 9857190). PTPN7, via negative regulation of MAPK signalling, plays a key role in T-cell and B-cell development and is the only known PTP whose expression in T-lymphocytes is induced by IL-2 (PMID: 15980098). PTPN7 is a 38 kDa non-receptor PTP expressed predominately in leukocytes, spleen and thymus (PMID: 1530918, 24265715, 19636879).

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