Anticorps Monoclonal anti-PTPN9

• Phare
• Validé par KD/KO

PTPN9 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D6E4

N° de cat : 67931-1-Ig

Synonymes

MEG2, PTPase MEG2, PTPMEG2, PTPN9

---

Galerie de données de validation

• 

  WB analysis using 67931-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67931-1-Ig (PTPN9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67931-1-Ig

  WB result of PTPN9 antibody (67931-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN9 transfected A549 cells.

• 

  WB analysis of pig pancreas using 67931-1-Ig

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 67931-1-Ig (PTPN9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67931-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67931-1-Ig (PTPN9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 67931-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PTPN9 antibody (67931-1-Ig, Clone: 1D6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules HeLa, cellules Jurkat, cellules RAW 264.7, tissu pancréatique de porc
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67931-1-Ig cible PTPN9 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTPN9 Protéine recombinante Ag30615
• Nom complet: protein tyrosine phosphatase, non-receptor type 9
• Masse moléculaire calculée: 593 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC010863
• Symbole du gène: PTPN9
• Identification du gène (NCBI): 5780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177).

{{ptg:RelatedPrimaryAntibodies}}