Anticorps Monoclonal anti-PTPRH

PTPRH Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3C6E8

N° de cat : 68635-1-Ig

Synonymes

3C6E8, R PTP H, SAP 1, SAP1

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Galerie de données de validation

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  WB analysis of pig spleen using 68635-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 68635-1-Ig (PTPRH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 68635-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68635-1-Ig (PTPRH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HT-29 using 68635-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using PTPRH antibody (68635-1-Ig, Clone: 3C6E8 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Applications testées

• Résultats positifs en WB: tissu splénique de porc, cellules A431
• Résultats positifs en IF/ICC: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68635-1-Ig cible PTPRH dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTPRH Protéine recombinante Ag17351
• Nom complet: protein tyrosine phosphatase, receptor type, H
• Masse moléculaire calculée: 1115 aa, 122 kDa
• Poids moléculaire observé: 200-210 kDa
• Numéro d’acquisition GenBank: BC111716
• Symbole du gène: PTPRH
• Identification du gène (NCBI): 5794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPRH, also known as SAP-1 (Stomach Cancer-Associated Protein-Tyrosine Phosphatase-1), is a member of the protein tyrosine phosphatase (PTP) family. These enzymes regulate cellular signaling by dephosphorylating tyrosine residues on proteins, counterbalancing kinase activity (PMID: 11278335). PTPRH is classified as a receptor-type PTP, characterized by an extracellular domain, a transmembrane region, and intracellular catalytic domains. In colorectal, gastric, and hepatocellular cancers, PTPRH is often upregulated and linked to poor prognosis (PMID: 12101188). Paradoxically, it may suppress tumorigenesis in other contexts by stabilizing cell adhesion. Overexpression in certain cancers suggests utility as a diagnostic or prognostic marker. The molecular weight of PTPRH is 122 kDa, it is reported that it can form a dimer with a molecular weight of 210 kDa (PMID:15850787).

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