Anticorps Polyclonal de lapin anti-PTPRJ

• Phare
• Validé par KD/KO

PTPRJ Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55123-1-PBS

Synonymes

DEP-1, DEP1, DEP 1, Density-enhanced phosphatase 1, Density enhanced phosphatase 1

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Galerie de données de validation

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  WB analysis of multi-cells/tissue using 55123-1-AP (same clone as 55123-1-PBS)

  WB result of 55123-1-AP. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 55123-1-AP (same clone as 55123-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  WB analysis using 55123-1-AP (same clone as 55123-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55123-1-AP (same clone as 55123-1-PBS)

  WB result of PTPRJ antibody (55123-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRJ transfected HeLa cells. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55123-1-AP (same clone as 55123-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55123-1-PBS cible PTPRJ dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein tyrosine phosphatase, receptor type, J
• Masse moléculaire calculée: 146 kDa
• Poids moléculaire observé: 146-170 kDa
• Numéro d’acquisition GenBank: NM_002843
• Symbole du gène: PTPRJ
• Identification du gène (NCBI): 5795
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTPRJ, also named as CD148 and DEP1, belongs to the protein-tyrosine phosphatase family and Receptor class 3 subfamily. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRJ may contribute to the mechanism of contact inhibition of cell growth. PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 (PLCG1) and Linker for Activation of T Cells (LAT). It was also found to dephosphorylate PDGF beta receptor, and may be involved in UV-induced signal transduction. The antibody is specific to PTPRJ.

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