Anticorps Monoclonal anti-PTPRO

PTPRO Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F2B4

N° de cat : 67000-1-PBS

Synonymes

2F2B4, EC:3.1.3.48, GLEPP1, PTP U2, PTPase U2

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Galerie de données de validation

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  WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67000-1-Ig (PTPRO antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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  WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67000-1-Ig (PTPRO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67000-1-PBS cible PTPRO dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTPRO Protéine recombinante Ag8284
• Nom complet: protein tyrosine phosphatase, receptor type, O
• Masse moléculaire calculée: 138 kDa
• Poids moléculaire observé: 160 kDa, 180-220 kDa
• Numéro d’acquisition GenBank: BC035960
• Symbole du gène: PTPRO
• Identification du gène (NCBI): 5800
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).

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