Anticorps Recombinant de lapin anti-PTRF

PTRF Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E15

N° de cat : 82697-1-PBS

Synonymes

FKSG13, Cavin-1, Cavin 1, 3E15

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Galerie de données de validation

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  WB analysis using 82697-1-RR (same clone as 82697-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

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  WB analysis of rat lung using 82697-1-RR (same clone as 82697-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82697-1-RR (same clone as 82697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82697-1-RR (PTRF antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82697-1-RR (same clone as 82697-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (82697-1-RR, Clone: 3E15 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82697-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82697-1-RR against Human PTRF were performed. The affinity constant is 0.303 nM.

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Informations sur le produit

82697-1-PBS cible PTRF dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PTRF Protéine recombinante Ag13486
• Nom complet: polymerase I and transcript release factor
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC008849
• Symbole du gène: PTRF
• Identification du gène (NCBI): 284119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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