Anticorps Recombinant de lapin anti-PUM1

PUM1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250792G4

N° de cat : 86129-1-RR

Synonymes

HsPUM, KIAA0099, PUMH, PUMH1, Pumilio 1

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Galerie de données de validation

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  WB analysis of rat brain using 86129-1-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 86129-1-RR (PUM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86129-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86129-1-RR (PUM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86129-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PUM1 antibody (86129-1-RR, Clone: 250792G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86129-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86129-1-RR against Human PUM1 were performed. The affinity constant is 2.46 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules HEK-293, cellules K-562, cellules NIH/3T3, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86129-1-RR cible PUM1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PUM1 Protéine recombinante Ag24651
• Nom complet: pumilio homolog 1 (Drosophila)
• Masse moléculaire calculée: 1186 aa, 126 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC013398
• Symbole du gène: PUM1
• Identification du gène (NCBI): 9698
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PUM1, also named as Pumilio homolog 1, is a 1186 amino acid protein, which is expressed in brain, heart, kidney, muscle, intestine and stomach. PUM1 as sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). The calculated molecular weight of PUM1 is 126 kDa, but the modified PUM1 is about 130-140 kDa.

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