Anticorps Polyclonal de lapin anti-PUS1

• Phare
• Validé par KD/KO

PUS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11512-1-PBS

Synonymes

EC:5.4.99.-, EC:5.4.99.12, MLASA, PP8985, pseudouridylate synthase 1

---

Galerie de données de validation

• 

  WB analysis of mouse lung using 11512-1-AP (same clone as 11512-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11512-1-AP (same clone as 11512-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11512-1-AP (same clone as 11512-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 11512-1-AP (same clone as 11512-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PUS1 antibody (11512-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

11512-1-PBS cible PUS1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PUS1 Protéine recombinante Ag2071
• Nom complet: pseudouridylate synthase 1
• Masse moléculaire calculée: 427 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC019320
• Symbole du gène: PUS1
• Identification du gène (NCBI): 80324
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}