Anticorps Polyclonal de lapin anti-CD155/PVR

• Phare
• Validé par KD/KO

CD155/PVR Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 27486-1-AP

Synonymes

PVR, Nectin like protein 5, NECL-5, NECL5, Necl 5

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Galerie de données de validation

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  WB analysis of A549 using 27486-1-AP

  WB result of CD155/PVR antibody (27486-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CD155/PVR knockout A549 cells.

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  WB analysis of HT-1080 using 27486-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (CD155/PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27486-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 27486-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (Poliovirus receptor antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 27486-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 27486-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules A549, cellules HUVEC
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

27486-1-AP cible CD155/PVR dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD155/PVR Protéine recombinante Ag26657
• Nom complet: poliovirus receptor
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC015542
• Symbole du gène: CD155/PVR
• Identification du gène (NCBI): 5817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

Publications

Species	Application	Title
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
		Acta Pharm Sin B; PKCα inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability; Authors - Jiaojiao Yu
human	IF	Sci Adv; Ara-C suppresses H3 K27-altered spinal cord diffuse midline glioma growth and enhances immune checkpoint blockade sensitivity; Authors - Bo Pang

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