Anticorps Monoclonal anti-CD155/PVR

CD155/PVR Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1F12A1

N° de cat : 66913-1-Ig

Synonymes

PVR, 1F12A1, CD155, HVED, Necl 5

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Galerie de données de validation

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  WB analysis using 66913-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66913-1-Ig (CD155/PVR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of K-562 using 66913-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66913-1-Ig (CD155/PVR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HT-1080, cellules HT-29, cellules K-562, cellules U-251

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• ELISA: See 1 publications below

Informations sur le produit

66913-1-Ig cible CD155/PVR dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD155/PVR Protéine recombinante Ag28303
• Nom complet: poliovirus receptor
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC015542
• Symbole du gène: CD155/PVR
• Identification du gène (NCBI): 5817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

Publications

Species	Application	Title
human	ELISA	iScience; Urinary extracellular vesicles signature for diagnosis of kidney disease; Authors - Keiichi Takizawa

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