Anticorps Polyclonal de lapin anti-Nectin-1/PVRL1

• Phare
• Validé par KD/KO

Nectin-1/PVRL1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 24713-1-AP

Synonymes

CD111, CLPED1, ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, HIgR, HVEC, nectin 1, Nectin-1, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK 12

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Galerie de données de validation

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  WB analysis using 24713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24713-1-AP (Nectin-1/PVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 24713-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24713-1-AP (Nectin-1/PVRL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 24713-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 24713-1-AP (Nectin-1/PVRL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 24713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24713-1-AP (Nectin-1/PVRL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24713-1-AP (Nectin-1/PVRL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 24713-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 24713-1-AP (Nectin-1/PVRL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, cellules SKOV-3, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

24713-1-AP cible Nectin-1/PVRL1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nectin-1/PVRL1 Protéine recombinante Ag20074
• Nom complet: poliovirus receptor-related 1 (herpesvirus entry mediator C)
• Masse moléculaire calculée: 517 aa, 57 kDa
• Poids moléculaire observé: 90 kDa, 57 kDa
• Numéro d’acquisition GenBank: BC104948
• Symbole du gène: PVRL1
• Identification du gène (NCBI): 5818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nectins are a family of immunoglobulin-like cell adhesion molecules comprising four members, nectin-1 to nectin-4, each consisting of an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain (PMID: 12901789, 18703497). Nectins are involved in cell adhesion, migration, polarization, morphogenesis, proliferation, differentiation, and survival. Nectin-1, also known as PVRL1, HVEC and CD111, is widely expressed in a variety of cell types including epithelial cells, neurons, and fibroblasts (PMID: 12901789). It promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Nectin-1 acts as a receptor for several alphaherpesviruses, including herpes simplex viruses (HSV) 1 and 2, porcine pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1) (PMID: 9616127). Nectin-1 is a glycoprotein, the glycosylated form has a larger apparent molecular weight than the calculated molecular weight of 57 kDa (PMID: 12376527; 15731277).

Publications

Species	Application	Title
human	WB	PLoS Pathog; Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1.; Authors - Hiroki Takeuchi
human	IHC,WB	Front Oncol; High expression of nectin-1 indicates a poor prognosis and promotes metastasis in hepatocellular carcinoma; Authors - Xuequan Wang ; KD Validated
mouse	IHC	Mol Ther Oncolytics; Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma.; Authors - Gaku Takano
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen
pig	WB,IF	J Virol; TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication; Authors - Xiu-Qing Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Morgan (Verified Customer) (08-09-2021)

This PVRL1 worked well with the ovarian cancer cell line OV8. It was more difficult to get a clear detection in the ovarian cancer cell line OV90, but we believe a similar trend was apparent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: OV8 and 0V90

Nectin-1/PVRL1 Antibody Western Blot validation (1:1000 dilution) in OV8 and 0V90 (Cat no:24713-1-AP)