Anticorps Polyclonal de lapin anti-Nectin 3

Nectin 3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 11213-1-AP

Synonymes

Nectin-3, PVRL3, CD113, Nectin cell adhesion molecule 3, poliovirus receptor related 3

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Galerie de données de validation

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  WB analysis using 11213-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11213-1-AP (Nectin-3/PVRL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11213-1-AP

  IP result of anti-Nectin-3/PVRL3 (IP:11213-1-AP, 4ug; Detection:11213-1-AP 1:600) with mouse testis tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 11213-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11213-1-AP (Nectin-3/PVRL3 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

11213-1-AP cible Nectin 3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nectin 3 Protéine recombinante Ag1712
• Nom complet: poliovirus receptor-related 3
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC017572
• Symbole du gène: Nectin-3
• Identification du gène (NCBI): 25945
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nectin 3, also known as CD113, is a member of the nectin family of cell adhesion molecules. It plays a crucial role in cell-cell adhesion and the formation of adherens junctions, which are essential for maintaining tissue integrity and cell polarity. Nectin 3 can form homophilic and heterophilic interactions, meaning it can bind to itself or other nectin family members, such as Nectin-2 (PMID: 10744716; 22902367). These interactions are important for processes like cell migration, tissue development, and immune cell transmigration (PMID: 12901789; 24116228).

Publications

Species	Application	Title
human	WB	Cancer Commun (Lond); ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin-3 and NRXN3.; Authors - Yin Zhao
human	WB	Front Microbiol; Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.; Authors - Dennis Schöttelndreier
rat,mouse	WB,IF	J Neurochem; Characterization of nectin processing mediated by presenilin-dependent γ-secretase.; Authors - Kim Jinsook J
human	IHC	Biomed Pharmacother; Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells.; Authors - Feixue Xu
mouse	IF	Biol Reprod; Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.; Authors - Przemysław Zakrzewski
human	WB,IF	Nat Chem Biol; Photoproximity labeling of endogenous receptors in the live mouse brain in minutes; Authors - Mikiko Takato

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgan (Verified Customer) (08-09-2021)

This PVRL3 antibody worked wonderfully with 2 ovarian cancer cell lines; OV8 and OV90. I was very pleased with the results.

• Applications: Western Blot
• Cell Tissue Type: OV8 and 0V90

Nectin 3 Antibody Western Blot validation ( dilution) in OV8 and 0V90 (Cat no:11213-1-AP)

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