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Anticorps Polyclonal de lapin anti-PWP1

PWP1 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

N° de cat : 14767-1-AP

Synonymes

IEF SSP 9502, PWP1, PWP1 homolog (S. cerevisiae)

Galerie de données de validation

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  • Western Blot (WB) analysis of Jurkat cells using PWP1 Polyclonal antibody (14767-1-AP)

    WB analysis of Jurkat using 14767-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14767-1-AP (PWP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PWP1 Polyclonal antibody (14767-1-AP)

    WB analysis of human liver using 14767-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14767-1-AP (PWP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PWP1 Polyclonal antibody (14767-1-AP)

    WB analysis of Jurkat using 14767-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14767-1-AP (PWP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules Jurkat, tissu hépatique humain

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

14767-1-AP cible PWP1 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PWP1 Protéine recombinante Ag6533
Nom complet PWP1 homolog (S. cerevisiae)
Masse moléculaire calculée 56 kDa
Poids moléculaire observé 50-56 kDa
Numéro d’acquisition GenBankBC001652
Symbole du gène PWP1
Identification du gène (NCBI) 11137
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Protocole

Product Specific Protocols
WB protocol for PWP1 antibody 14767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

H (Verified Customer) (04-05-2020)

There are quite lots of background bands. However, I could detect which is the target band through nuclear-cytosolic fractionation under starvation condition, during which, PWP1 translocate from nucleus to cytosol.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
PWP1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14767-1-AP)