Anticorps Polyclonal de lapin anti-PWWP2B

PWWP2B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 32112-1-AP

Synonymes

PWWP Domain Containing 2, PWWP Domain Containing 2B , PWWP domain-containing protein 2B, PWWP2

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Galerie de données de validation

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  WB analysis using 32112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32112-1-AP (PWWP2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules BxPC-3, cellules HaCaT, cellules HeLa, cellules LNCaP

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32112-1-AP cible PWWP2B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PWWP2B Protéine recombinante Ag36839
• Nom complet: PWWP domain containing 2B
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC068574
• Symbole du gène: PWWP2B
• Identification du gène (NCBI): 170394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PWWP2B (PWWP structural domain protein 2B) is a PWWP structural domain-containing protein involved in a variety of cellular functions and biological processes. PWWP2B is highly expressed in brown and beige adipose tissue and its expression is induced by cold stimulation. It has been shown that PWWP2B negatively regulates adipose tissue thermogenesis by recruiting and stabilizing the histone deacetylase HDAC1/2, which binds to the promoter regions of thermogenic genes and reduces histone acetylation levels. In adipocytes, PWWP2B acts as part of the nucleosome remodeling and deacetylation complex (NuRD) to inhibit the expression of thermogenic genes and avoid overactivation of thermogenic functions. In addition, PWWP2B plays an important role in DNA damage repair, as its PWWP structural domain moves to DNA damage sites, interacts with UHRF1 and recruits MRE11 to promote DNA end resection and homologous recombination repair. In pathology, downregulation of PWWP2B is associated with recurrent gastric cancer and promotes cell proliferation and migration.

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