Anticorps Monoclonal anti-PXR

PXR Monoclonal Antibody for FC (Intra), IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

1G3A7

N° de cat : 67912-1-Ig

Synonymes

BXR, NR1I2, ONR1, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, PAR, PAR1, PAR2, PARq, Pregnane X receptor, PRR, PXR, SAR, SXR

---

Galerie de données de validation

• 

  WB analysis using 67912-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67912-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67912-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 67912-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 67912-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MCF-7 using 67912-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PXR (67912-1-Ig, Clone:1G3A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules COLO 320, cellules MCF-7, tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

67912-1-Ig cible PXR dans les applications de WB, IP, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PXR Protéine recombinante Ag7991
• Nom complet: nuclear receptor subfamily 1, group I, member 2
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC017304
• Symbole du gène: NR1I2
• Identification du gène (NCBI): 8856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, plays a key role in the induction of genes involved in drug transport and metabolism. It modulates drug transport and metabolism through the regulation of target genes responsible for the transport and conversion of chemicals into metabolites that are more easily eliminated from the body [PMID: 22609277]. It is also emerging as an endobiotic receptor that regulates the inflammatory response. In addition, PXR acts as a transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds [PMID: 19297428].

Publications

Species	Application	Title
human	WB	Front Pharmacol; Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway; Authors - Xiaowei Sun
human	WB,IF,IP	Phytomedicine; Panaxytriol upregulates CYP3A4 expression based on the interaction of PXR, CAR, HSP90α, and RXRα.; Authors - Lingming Zhang
mouse	WB	Food Funct; CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling.; Authors - Xin Shen
human	IF	J Ethnopharmacol; Panaxytriol upregulates CYP3A4 expression through the interaction between nuclear regulators and DNA response elements; Authors - Jingdi Yan
mouse	WB	Part Fibre Toxicol; Type 1 diabetes and diet-induced obesity predispose C57BL/6J mice to PM2.5-induced lung injury: a comparative study; Authors - Shen Chen
mouse	WB	Acta Pharmacol Sin; Pregnane X receptor activation alleviates renal fibrosis in mice via interacting with p53 and inhibiting the Wnt7a/β-catenin signaling; Authors - Wen-Hua Ming