Anticorps Polyclonal de lapin anti-ASC/TMS1

ASC/TMS1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 30641-1-AP

Synonymes

PYCARD, PYD and CARD domain containing, masc, CARD5, Asc

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Galerie de données de validation

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  WB analysis using 30641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30641-1-AP (PYCARD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30641-1-AP (PYCARD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 30641-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PYCARD antibody (30641-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, tissu de thymus de rat, tissu de thymus de souris, tissu splénique de rat
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

30641-1-AP cible ASC/TMS1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASC/TMS1 Protéine recombinante Ag32519
• Nom complet: PYD and CARD domain containing
• Masse moléculaire calculée: 21KD
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: NM_023258
• Symbole du gène: Pycard
• Identification du gène (NCBI): 66824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).

Publications

Species	Application	Title
mouse	WB	Food Sci Biotechnol; Geraniol alleviates liver injury induced by bisphenol A via modulating NLRP3/caspase-1 pathway and gut microbiota in mice model; Authors - Bangyuan Zhou
human,mouse	WB	Int Immunopharmacol; Deletion of BRCC3 ameliorates airway inflammation in asthma by inhibiting the activation of NLRP3 inflammasome; Authors - Hao Wang
mouse	WB	Tissue Cell; Baicalin reduced vandetanib induced myocardial injury by regulating redox balance and NLRP3 inflammasome pathway; Authors - Fen Wang
rat	WB	Front Pharmacol; Edaravone dexborneol protected neurological function by targeting NRF2/ARE and NF-κB/AIM2 pathways in cerebral ischemia/reperfusion injury; Authors - Hui Zhang

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