Anticorps Recombinant de lapin anti-ASC/TMS1

ASC/TMS1 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240908D5

N° de cat : 83858-3-PBS

Synonymes

Pycard, 240908D5, Apoptosis-associated speck-like protein containing a CARD, Asc, CARD5

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Galerie de données de validation

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  WB analysis using 83858-3-RR (same clone as 83858-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83858-3-RR (PYCARD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83858-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 83858-3-RR (same clone as 83858-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83858-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 83858-3-RR (same clone as 83858-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83858-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83858-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83858-3-RR against Mouse PYCARD were performed. The affinity constant is 3.58 nM.

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Informations sur le produit

83858-3-PBS cible ASC/TMS1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ASC/TMS1 Protéine recombinante Ag32519
• Nom complet: PYD and CARD domain containing
• Masse moléculaire calculée: 21KD
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: NM_023258
• Symbole du gène: Pycard
• Identification du gène (NCBI): 66824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).

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