Anticorps Monoclonal anti-PYCR1

PYCR1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H2G1

N° de cat : 66510-1-PBS

Synonymes

P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2

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Galerie de données de validation

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  WB analysis using 66510-1-Ig (same clone as 66510-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66510-1-Ig (PYCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1 hour. This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66510-1-Ig (same clone as 66510-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR1 antibody (66510-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50786-1

  Cytometric bead array standard curve of MP50786-1, PYCR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66510-2-PBS. Detection antibody: 66510-1-PBS. Standard:Ag17919. Range: 0.098-100 ng/mL.

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Informations sur le produit

66510-1-PBS cible PYCR1 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PYCR1 Protéine recombinante Ag17919
• Nom complet: pyrroline-5-carboxylate reductase 1
• Masse moléculaire calculée: 319 aa, 33.8 kDa
• Poids moléculaire observé: 33 kDa, 35 kDa
• Numéro d’acquisition GenBank: BC022244
• Symbole du gène: PYCR1
• Identification du gène (NCBI): 5831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.

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