Anticorps Monoclonal anti-PYGL

• Phare
• Validé par KD/KO

PYGL Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G12D8

N° de cat : 66769-1-PBS

Synonymes

Glycogen phosphorylase, liver form, EC:2.4.1.1, 1G12D8

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Galerie de données de validation

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  WB analysis of HSC-T6 using 66769-1-Ig (same clone as 66769-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

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  WB analysis using 66769-1-Ig (same clone as 66769-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66769-1-Ig (same clone as 66769-1-PBS)

  WB result of PYGL antibody (66769-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGL transfected HeLa cells. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 66769-1-Ig (same clone as 66769-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PYGL antibody (66769-1-Ig, Clone: 1G12D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66769-1-PBS cible PYGL dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PYGL Protéine recombinante Ag8550
• Nom complet: phosphorylase, glycogen, liver
• Masse moléculaire calculée: 846 aa, 97 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC009895
• Symbole du gène: PYGL
• Identification du gène (NCBI): 5836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glycogen phosphorylase L (PYGL) is one of the gene related to hypoxia metabolism and was found to be up-regulated in head and neck squamous cell carcinomas (HNSCCs) and breast cancers. Glycogen synthases (GSs) and glycogen phosphorylases (GPs) catalyze the key steps of glycogen synthesis and breakdown, while glycogen phosphorylase has three isoforms: PYGM (muscle), PYGL (liver), and PYGB (brain) (PMID: 37063425, 9529348). High PYGL expression plays an independent role in predicting the poor prognosis of glioma patients (PMID: 34177761).

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