Anticorps Polyclonal de lapin anti-PYGO2

• Phare
• Validé par KD/KO

PYGO2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 11555-1-AP

Synonymes

PYGO 2, PP7910

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Galerie de données de validation

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  WB analysis of MDA-MB-453s using 11555-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 11555-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 11555-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 11555-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11555-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11555-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11555-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11555-1-AP (PYGO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-453s, cellules C6, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

11555-1-AP cible PYGO2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PYGO2 Protéine recombinante Ag2111
• Nom complet: pygopus homolog 2 (Drosophila)
• Masse moléculaire calculée: 406 aa, 41 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC032099
• Symbole du gène: PYGO2
• Identification du gène (NCBI): 90780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PYGO2, also named as Pygopus homolog 2, is a 406 animo acid protein, which contains 1 PHD-type zinc finger. PYGO2 is involved in signal transduction through the Wnt pathway and Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex. PYGO2 is in a high proportion of breast and epithelial ovarian malignant tumors and is required for the growth of several cell lines derived from these carcinomas.

Publications

Species	Application	Title
human	WB,IHC	J Cancer; Pygo2 as a novel biomarker in gastric cancer for monitoring drug resistance by upregulating MDR1.; Authors - Dongdong Zhang ; KD Validated
human	WB	Nat Commun; Structural basis of the interaction between BCL9-Pygo and LDB-SSBP complexes in assembling the Wnt enhanceosome; Authors - Hongyang Wang

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