Anticorps Recombinant de lapin anti-PYROXD1

• Phare
• Validé par KD/KO

PYROXD1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241639D7

N° de cat : 84289-7-RR

Synonymes

241639D7, pyridine nucleotide disulphide oxidoreductase domain 1

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Galerie de données de validation

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  WB analysis of HeLa using 84289-7-RR

  WB result of PYROXD1 antibody (84289-7-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYROXD1 transfected HeLa cells.

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  WB analysis using 84289-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84289-7-RR (PYROXD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84289-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84289-7-RR against Human PYROXD1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Caco-2, cellules COLO 320, cellules MG U-87, cellules PC-3, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84289-7-RR cible PYROXD1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PYROXD1 Protéine recombinante Ag34768
• Nom complet: pyridine nucleotide-disulphide oxidoreductase domain 1
• Poids moléculaire observé: 55~60 kDa
• Numéro d’acquisition GenBank: BC021662
• Symbole du gène: PYROXD1
• Identification du gène (NCBI): 79912
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

pyridine nucleotide-disulfide oxidoreductase domain 1 (PYROXD1) is a putative oxidoreductase that co-evolved with the tRNA-LC and is essential for its activity in cells and in vivo. PYROXD1 oxidizes NAD(P)H to NAD(P)+ with tightly controlled kinetics, reducing one molecule of O2 into H2O2 per turnover. PYROXD1 is expressed across a range of tissue types in humans, including skeletal muscle, and is conserved across many species.

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