Anticorps Recombinant de lapin anti-Pan-Keratin (Type I)

Pan-Keratin (Type I) Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251039C1

N° de cat : 86238-2-PBS

Synonymes

pan-CK, Pan-CK (Type I), Pan-Keratin, Type I keratin, Type I Keratin (Acidic)

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Galerie de données de validation

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  WB analysis using 86238-2-RR (same clone as 86238-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human cervical cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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Informations sur le produit

86238-2-PBS cible Pan-Keratin (Type I) dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Pan-Keratin (Type I)
• Poids moléculaire observé: 45-65 kDa
• Identification du gène (NCBI): Pan-Keratin (Type I)
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40.

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