Anticorps Recombinant de lapin anti-Pan-Keratin (Type I)

Pan-Keratin (Type I) Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

251039C1

N° de cat : 86238-2-RR

Synonymes

pan-CK, Pan-CK (Type I), Pan-Keratin, Type I keratin, Type I Keratin (Acidic)

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Galerie de données de validation

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  WB analysis using 86238-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human cervical cancer using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skin using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HaCaT, cellules A431, cellules HT-29, tissu cutané de rat, tissu cutané de souris, tissu de côlon de rat
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu amygdalien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du col de l'utérus humain, tissu cutané de souris, tissu de cancer du côlon humain, tissu de cancer du poumon humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86238-2-RR cible Pan-Keratin (Type I) dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Pan-Keratin (Type I)
• Poids moléculaire observé: 45-65 kDa
• Identification du gène (NCBI): Pan-Keratin (Type I)
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40.

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