Anticorps Recombinant de lapin anti-Phospho-Tyrosine

Phospho-Tyrosine Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

-

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250495G7

N° de cat : 86398-1-RR

Synonymes

p Tyrosine, Pan-phospho-Tyr, Phospho Tyrosine, Phospho-Tyrosine (P-Tyr-1000), P-Tyr-1000

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Galerie de données de validation

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  WB analysis using 86398-1-RR

  Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 86398-1-RR (Phospho-Tyrosine antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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  IF Staining of A431 using 86398-1-RR

  Immunofluorescent analysis of (4% PFA) fixed hEGF6 treated A431 cells using Phospho-Tyrosine antibody (86398-1-RR, Clone: 250495G7 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: EGF treated A431 cells,
• Résultats positifs en IF/ICC: hEGF6 treated A431 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86398-1-RR cible Phospho-Tyrosine dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons -

• Réactivité: -
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Phospho-Tyrosine
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phospho-Tyrosine antibody refers to a class of pan-specific antibodies or detection reagents that recognize all phosphorylated tyrosine residues regardless of the surrounding amino-acid sequence. These tools are widely used to monitor global tyrosine phosphorylation events in signal-transduction studies, cancer research and drug-discovery screens.
This antibody does not cross-react with peptides containing Phospho-Serine or Phospho-Threonine.

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