Anticorps Polyclonal de lapin anti-Pancreatic Polypeptide

Pancreatic Polypeptide Polyclonal Antibody for IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15493-1-PBS

Synonymes

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Galerie de données de validation

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  IHC staining of mouse pancreas using 15493-1-AP (same clone as 15493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15493-1-AP (Pancreatic Polypeptide antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 15493-1-AP (same clone as 15493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15493-1-AP (Pancreatic Polypeptide antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

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  IF Staining of rat pancreas using 15493-1-AP (same clone as 15493-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Pancreatic Polypeptide antibody (15493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Pancreatic Polypeptide antibody (15493-1-AP, green) stained on PP cells in rat pancreas islet. Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red) stained on α cells in rat pancreas islet. This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

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  IF Staining of rat pancreas using 15493-1-AP (same clone as 15493-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Pancreatic Polypeptide antibody (15493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Pancreatic Polypeptide antibody (15493-1-AP) (green) stained on PP cells in rat pancreas islet. Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red) stained on α cells in rat pancreas islet. This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 15493-1-AP (same clone as 15493-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Pancreatic Polypeptide (15493-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15493-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15493-1-PBS cible Pancreatic Polypeptide dans les applications de IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Pancreatic Polypeptide Protéine recombinante Ag7886
• Nom complet: pancreatic polypeptide
• Masse moléculaire calculée: 10 kDa
• Numéro d’acquisition GenBank: BC032225
• Symbole du gène: Pancreatic Polypeptide
• Identification du gène (NCBI): 5539
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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