Anticorps Polyclonal de lapin anti-Parvalbumin

Parvalbumin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 29312-1-AP

Synonymes

PVALB, Parvalbumin alpha

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Galerie de données de validation

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  WB analysis using 29312-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29312-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29312-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum tissue using 29312-1-AP

  Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:1500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University)

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris, tissu de cervelet de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cervelet de souris, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

29312-1-AP cible Parvalbumin dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Parvalbumin Protéine recombinante Ag30189
• Nom complet: parvalbumin
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: NM_002854
• Symbole du gène: Parvalbumin
• Identification du gène (NCBI): 5816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).

Publications

Species	Application	Title
mouse	WB,IHC,IF	Front Mol Neurosci; PGC-1α regulates critical period onset/closure, mediating cortical plasticity; Authors - Wei-Jun Zhang
rat	IF	Physiol Res; High-Intensity Interval Training Increases Osteoarthritis-Associated Pain-Sensitive Threshold Through Reduction of Perineuronal Nets of the Medial Prefrontal Cortex in Rats; Authors - X Zhang
rat	IHC	Transl Androl Urol; Whole body vibration training promotes proprioceptive pathway for the treatment of stress urinary incontinence in rats; Authors - Chengyu Zhou
mouse	WB	Mol Neurobiol; Oxidative Stress-mediated Loss of Hippocampal Parvalbumin Interneurons Contributes to Memory Precision Decline After Acute Sleep Deprivation; Authors - Yu-Zhu Gao
mouse	IF	Cell Rep Methods; A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution; Authors - Jingtan Zhu
mouse	IF	STAR Protoc; Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID; Authors - Jingtan Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

Parvalbumin (red) antibody worked in my human brain cortex sections, although it marks mostly the synaptic terminals more than the soma of the parvalbumin cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Parvalbumin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:29312-1-AP)

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