Anticorps Polyclonal de lapin anti-Parvalbumin

Parvalbumin Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29312-1-PBS

Synonymes

PVALB, Parvalbumin alpha

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Galerie de données de validation

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  WB analysis using 29312-1-AP (same clone as 29312-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

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  WB analysis using 29312-1-AP (same clone as 29312-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29312-1-AP (same clone as 29312-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29312-1-AP (Parvalbumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 29312-1-AP (same clone as 29312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29312-1-AP (Parvalbumin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum tissue using 29312-1-AP (same clone as 29312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Parvalbumin antibody (29312-1-AP) at dilution of 1:1500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 29312-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29312-1-PBS cible Parvalbumin dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Parvalbumin Protéine recombinante Ag30189
• Nom complet: parvalbumin
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: NM_002854
• Symbole du gène: Parvalbumin
• Identification du gène (NCBI): 5816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).

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