Anticorps Recombinant de lapin anti-Parvalbumin

Parvalbumin Recombinant Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250158B6

N° de cat : 85819-4-PBS

Synonymes

PVALB, Parvalbumin alpha

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Galerie de données de validation

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  IHC staining of mouse cerebellum using 85819-4-RR (same clone as 85819-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85819-4-RR (PVALB antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85819-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 85819-4-RR (same clone as 85819-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85819-4-RR (PVALB antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85819-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 85819-4-RR (same clone as 85819-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Parvalbumin antibody (85819-4-RR, Clone: 250158B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85819-4-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 85819-4-RR (same clone as 85819-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Parvalbumin antibody (85819-4-RR, Clone: 250158B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85819-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85819-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85819-4-RR against Human Parvalbumin were performed. The affinity constant is 0.715 nM.

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Informations sur le produit

85819-4-PBS cible Parvalbumin dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Parvalbumin Protéine recombinante Ag30189
• Nom complet: parvalbumin
• Masse moléculaire calculée: 12 kDa
• Numéro d’acquisition GenBank: NM_002854
• Symbole du gène: Parvalbumin
• Identification du gène (NCBI): 5816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).

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