Anticorps Polyclonal de lapin anti-Perforin

Perforin Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 14580-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14580-1-AP (same clone as 14580-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14580-1-AP (Perforin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14580-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 14580-1-AP (same clone as 14580-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14580-1-AP (PRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14580-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14580-1-PBS cible Perforin dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Perforin Protéine recombinante Ag6060
• Nom complet: perforin 1 (pore forming protein)
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC063043
• Symbole du gène: Perforin
• Identification du gène (NCBI): 5551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Perforin 1 (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)

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