Anticorps Recombinant de lapin anti-Perforin

Perforin Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241009C10

N° de cat : 83977-1-PBS

Synonymes

241009C10, Cytolysin, FLH2, HPLH2, Lymphocyte pore-forming protein

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Galerie de données de validation

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  WB analysis using 83977-1-RR (same clone as 83977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83977-1-RR (Perforin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83977-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00872-1

  Cytometric bead array standard curve of MP00872-1, Perforin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83977-2-PBS. Detection antibody: 83977-1-PBS. Standard: Ag6060. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83977-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83977-1-PBS against Human Perforin was performed. The affinity constant is 1.33 nM.

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  Affinity and Kinetic Characterization of 83977-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83977-1-RR against Human Perforin were performed. The affinity constant is 1.05 nM.

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Informations sur le produit

83977-1-PBS cible Perforin dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Perforin Protéine recombinante Ag6060
• Nom complet: perforin 1 (pore forming protein)
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC063043
• Symbole du gène: Perforin
• Identification du gène (NCBI): 5551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Perforin (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)

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